SUMOylation is elevated in human anterior subcapsular cataract (ASC) and drives the epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs). (IMAGE)
Caption
(A) Representative anterior segment photographs of transparent capsules and ASC plaques in human patients. Scale bar: 2 mm.
(B) Immunofluorescence staining of lens epithelium from ASC patients and controls. SUMO1 (red), α-SMA (green), and DAPI (nuclear counterstain, blue). Scale bar: 50 μm.
(C) Quantification analysis of SUMO1 and α-SMA fluorescence intensity from (B). Two random regions per capsule were analyzed (n = 3 capsules/group). Statistical significance was determined by an unpaired Student's t-test. ***P < 0.001.
(D) Immunofluorescence co-staining of SUMO2/3 (red), α-SMA (green), and DAPI (nuclear, blue) in lens epithelium. Scale bar: 50 μm.
(E) Quantification of SUMO2/3 and α-SMA fluorescence intensity from (D). Data were analyzed as in (C). *P < 0.05 and **P < 0.01.
(F) Western blotting analysis of EMT markers in FHL124 LECs transfected with HA-tagged vector and SUMO isoforms. Cells were harvested 24 h post-transfection.
(G) Densitometric quantification of (F). Data were normalized to β-actin loading control. Statistical analysis was performed by one-way ANOVA with Bonferroni post-hoc test. **P < 0.01 and ***P < 0.001.
Credit
Min Hou, Yujie Ding, Xuan Bao, Liangping Liu, Yulan Wang, Mingxing Wu
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