Expression profile of USP32 in hepatocellular carcinoma (IMAGE)
Caption
(A) The expression of signature genes in hepatocellular carcinoma (HCC) was detected by analyzing the GSE94660 dataset, and a heatmap was generated. USP32 presented significantly high expression in HCC tissues, along with E2F7; (B) USP32 expression profiles relevant to HCC were detected via analysis of data from the TCGA database (n=421). USP32 was highly expressed in HCC tissues (P<0.0001); (C) Data analysis of the GEO94660 dataset and TCGA database revealed a significant positive correlation between USP32 and E2F7 (P<0.01). (D) Real-time quantitative polymerase chain reaction (RT‒qPCR) revealed that USP32 was significantly more highly expressed in HCC cell lines than in control THLE-2 cells (∗∗P<0.01); Western blot analysis revealed markedly greater expression of the USP32 protein in the three HCC cell lines than in the control cell line (P<0.01); (E) Statistics of the number of cases with high or low expression of USP32 in HCC specimens. USP32 was upregulated in most of the tumor tissues (75/95) and was expressed at a lower level in most of the adjacent noncancerous tissues (24/95) (P<0.01); (F) Representative images of the results of the immunohistochemistry analysis (400×) of HCC patient samples. The samples were used as controls and subjected to immunoglobulin G (IgG) antibody staining. USP32 expression in tumor tissues was significantly higher than that in adjacent noncancerous tissues.
Credit
Xin-yi Luo, Yi-quan Lu, Yi-fan Zhang, Nan Wang, Feng-jie Hao, Xiao-chun Fei, Yong-jun Chen, Jun-qing Wang.
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