Fig. 3. Biofilm formation ability via confocal laser-scanning microscopy (CLSM). (IMAGE)
Caption
Fig. 3. Biofilm formation ability via confocal laser-scanning microscopy (CLSM). (A) Analysis of biofilm formation ability of FNS sample using CLSM. (B) Analysis of biofilm formation ability of FNN sample using CLSM. (C) Analysis of biofilm formation ability of FNO sample using CLSM. (D) Biofilms of FNS, FNN, and FNO were cultured in 35-mm confocal dishes. Cells were stained with a Filmtracer (LIVE/DEAD Biofilm Viability Kit), and biofilm formation ability was detected using CLSM. Green fluorescence indicated live cells, and red fluorescence indicated dead cells. The maximum thickness of biofilm of FNS, FNN, and FNO was (44.16 ± 0.76) μm, (22.50 ± 0.20) μm, and (21.96 ± 0.15) μm, respectively. P < 0.001.
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