KAIST Develops a Genetic Switch That Works in Both Bacteria and Yeast (IMAGE)
Caption
Figure 1. Schematic of the design and functional validation of a gene control element (hybrid promoter) that operates in both yeast and bacteria
(Top left) The native promoter architectures of yeast and E. coli were analyzed to identify the core elements that control gene expression in each species. Structural features were compared, including the TATA box, transcription start site (TSS), 5′ untranslated region (5′ UTR) and Kozak sequence of the yeast promoter, and the UP element, −35 box, −10 box and Shine–Dalgarno sequence of the E. coli promoter.
(Top right) Rules for arranging and combining the yeast and E. coli promoter elements were established so that the elements remain functional across the two different transcriptional and translational systems. On this basis, the yeast core promoter region was joined in a single sequence with the E. coli −35 and −10 boxes and translation initiation elements, producing a hybrid promoter architecture that can operate in both yeast and bacteria.
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KAIST
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