NaCl disrupts skin barrier function in atopic dermatitis and suppresses keratinocyte differentiation. (IMAGE)
Caption
(A) Trans-epidermal water loss (TEWL) values of mice dorsal skin (n = 5) (B) Immunoblotting analysis of filaggrin (FLG) and loricrin (LOR) expression levels in skin lesions (C) Western blotting analysis of FLG and LOR expression levels at the indicated concentrations of NaCl in keratinocytes for 24 h (D) Western blotting analysis of FLG and LOR expression levels at different time points in keratinocytes treated with 20 mM NaCl (E) Quantitative reverse transcription PCR assay analysis of FLG and LOR mRNA expression levels in keratinocytes treated with different concentrations of NaCl (0 mM, 10 mM, 20 mM, 40 mM) (F) Quantitative reverse transcription PCR assay analysis of FLG and LOR mRNA expression levels at different time points in keratinocytes treated with 20 mM NaCl (G) Representative images of immuno-fluorescent staining of lysosome (stained by Lysotracker, red) and LOR or FLG (green) in keratinocytes treated with PBS and 20 mM NaCl. Scale bar: 10 μm (H) Representative immunohistochemistry images of skin lesions for LOR and FLG in PBS- or NaCl-treated mice. Scale bar: 50 μm. Data are shown as mean ± standard deviation from at least three independent experiments. Statistical analysis was performed using Student's t-test. Data are expressed as mean ± standard deviation. ∗∗p < 0.01 and ∗∗∗p < 0.001.
Credit
Yingqiang Luo, Zhiqiang Song, Pengju Jiang, Lan Ge, Min Zhang, Zihao Zhou, Yaguang Wu, Jun Hu
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