Early transcriptomic alterations reveal multiple-system dysregulation in pregnancies that develop PE. (IMAGE)
Caption
(A) Compared with control, women who later developed preterm PE had significantly higher GGT in early pregnancy. (B) Compared with control, women who later developed preterm PE had significantly higher TyG MoM in early pregnancy. (C) Volcano plot of DEGs (|log2 FC| > 0.2, P.adj < 0.05). The red indicates up-regulated genes and the blue indicates down-regulated genes between PE and control groups in the GEO datasets. (D) Intersection of GEO-derived placental tissue DEGs and mid-pregnancy cfDNA-derived DEGs. (E) GSEA analysis showed significant negative enrichment of pathways related to hepatic function (metabolism of xenobiotics by cytochrome P450, aspirin metabolism, drug metabolism cytochrome P450, glucuronidation, phase II conjugation of compounds) in PE cases. (F) GSEA analysis showed significant negative enrichment of pathways related to bile acid synthesis and clearance (disorders of bile acid synthesis and biliary transport, synthesis of bile acids and bile salts via 7 alpha hydroxycholesterol) in PE cases. (G) GSEA analysis showed significant negative enrichment of pathways related to lipid oxidation (oxysterols derived from cholesterol, fatty acid omega oxidation) in PE cases. (H) GSEA analysis showed significant up-regulation of pro-inflammatory signaling (IL-6 pathway, IL-1 structural pathway) in PE cases. (I) GSEA analysis showed that starch and sucrose metabolism pathways were significantly down-regulated, and insulin signaling pathways were significantly up-regulated in PE cases. (J) Differential promoter coverage of functionally relevant genes between control and preterm PE cases. PE, preeclampsia; GGT, gamma-glutamyltransferase; TyG MoM, triglyceride-glucose index multiple of the median; DEGs, differentially expressed genes; GSEA, gene set enrichment analysis; cfDNA, cell-free DNA; TSS, transcription start site. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.
Credit
Yi Zhu, Yanqiu Zhang, Chao Huang, Sheng Zhang, Jun Cao, Nicole Miranda, Sarina Zhao, Yan Peng , Chao Yu, Bin Feng, Jieyu Jin, Qingqin Tang, Jiaming Fan, Longwei Qiao, Yuting Liang
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND