Figure 1. Tumor suppressive functions of miR-766-5p (IMAGE)
Caption
(A) Western blot analysis of MYC in indicated cell lines after transfection with 10 nmol/L of miR-NC or miR-766-5p. The intensity of MYC bands was quantified by densitometry and shown as the fold change after normalization with β-actin. na, not applicable. (B) Western blot analysis of CBP and BRD4 in HCT116-/- cells 48 hours after transfection with 10 nmol/L of miR-NC, or miR-766-5p. (C) H3K27Ac Chromatin immunoprecipitation (ChIP)-qPCR at the indicated loci in HCT116-/- cells transfected with miR-NC or miR-766-5p. Red bars indicate super-enhancer regions of MYC. Relative ChIP enrichment values in the indicated regions are expressed as percentages relative to input DNA. Bar, SD for triplicate experiments. * P < 0.05. (D) Western blot analysis of BRD4-NUT, CBP, and MYC expression in Ty82-JQ1R cells transfected with 10 nmol/L of miR-NC or miR-766-5p. (E) Local administration of miR-766-5p suppressed in vivo tumor growth. (upper) Resected tumors at 21 days after the injection of Ty82-JQ1R cells. Scale bar, 10 mm. (lower) Tumor growth curves of xenograft mouse models treated with miR-NC or miR-766-5p (n = 5, each).
Credit
Department of Molecular Cytogenetics, TMDU
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