Experimental set-up for multimodal non-linear DCDC fiber scanning CARS/SHG/TPEF endoscopy (IMAGE)
Caption
a, Photo of the endomicroscopic fiber probe and laser coupling unit. A picture of the stainless-steel shielded probe head of 2.4 mm diameter and a length of 39 mm is shown in the upper right corner in comparison with the size of one Euro cent coin. The optical fiber and the four connection cables of the piezo-scanner are guided in a 1 mm tube, which is enclosed in a sealed medical grade endoscopic tube of 4.5 mm in diameter. b, The fiber laser (AFS, Germany) is collimated by the lens L1, filtered from FWM at the CARS wavelength by F1 (750 nm long pass). The laser power is adjusted using a 1050 nm short pass dichroic mirror (DC1). A linear diffractive grating G of 39.4 μm grating period and lens L3 (f = 4 mm) couple pump and Stokes into the two different cores. Sample signals are guided back through the DCDC-fiber and detected by the PMT-detector after deflection at the dichroic mirror DC2. The bandpass filter F2 is selecting the non-linear signal of interest (CARS/SHG/TPEF) and the lens L2 focusing the signals onto the photo multiplier tube (PMT).
Credit
by Ekaterina Pshenay-Severin, Hyeonsoo Bae, Karl Reichwald, Gregor Matz, Jörg Bierlich, Jens Kobelke, Adrian Lorenz, Anka Schwuchow, Tobias Meyer-Zedler, Michael Schmitt, Bernhard Messerschmidt, Juergen Popp
Usage Restrictions
Credit must be given to the creator.
License
CC BY