Multimodal CARS/SHG/TPEF endoscopic images of unstained biological samples (IMAGE)
Caption
First column CARS at 2850 cm⁻¹ in red, second column TPEF1 at 578 in green, third and fourth columns as indicated). a, Human epithelial tissue from head and neck location CARS, TPEF1 and TPEF2 at 458 nm and combined image, thickness 15 µm. Single epithelial cells and cell nuclei are resolved. b, CARS, TPEF, SHG and combined image of a dura mater section of ovis aries rich in collagen generating strong SHG signals, thickness 50 µm. c-e, CARS, TPEF and combined images from sections of Galleria mellonella larvae, thickness of 100 µm c) or 50 µm thickness d,e. For all endoscopic images the laser power @ sample: 10 mW for pump (795 nm), 32 mW for Stokes (1030 nm). FoV: 180 µm c and d and 70 µm a, b and e. LSM images were acquired for comparison at a Laser scanning microscope (LSM510, Zeiss, Germany) using 672.5 nm pump, 35 mW at sample, Stokes @ 832 nm, 40 mW at sample, 1-2 ps pulse duration, a repetition rate of 80 MHz and a 20×/0.8NA objective. The CARS signals were detected in forward direction after filtering by a 650 nm short pass filter and a 550nm/88 bandpass filter in contrast to the endomicroscopic probe. The TPEF signal was detected in epi-direction using a long pass 600 dichroic mirror, a 650 nm short pass filter and the band pass filter TPEF2. All scale bars are 10 µm, unless a different length is specified.
Credit
by Ekaterina Pshenay-Severin, Hyeonsoo Bae, Karl Reichwald, Gregor Matz, Jörg Bierlich, Jens Kobelke, Adrian Lorenz, Anka Schwuchow, Tobias Meyer-Zedler, Michael Schmitt, Bernhard Messerschmidt, Juergen Popp
Usage Restrictions
Credit must be given to the creator.
License
CC BY