new method yields better retinal sheet transplants (IMAGE)
Caption
Schematic showing the experimental results. Retinal sheets were grown from wild type human stem cells (left) or human stem cells missing the ISL1 gene (right). On the left, the transplanted photoreceptors (peach cells) are connected to transplanted bipolar cells (green) as part of the retinal sheet. On the right, the graft bipolar cells have died off, and the receptors connect to host bipolar cells (pink). The result is that all ganglion cells on the right respond to light, and responses are higher than those on the left. Photoreceptors (peach cells at the bottom) in the graft connect to bipolar cells (graft bipolar cells: green; host bipolar cells: pink). Host bipolar cells connect to host ganglion cells (top gray cells), which send axons to the brain as the optic nerve. recordings were made from the ganglion cells in response to light (red voltage trace).
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RIKEN
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