Dependence of fluorophore’s lifetime on chromatin replication timing (IMAGE)
Caption
(a) Representative fluorescence lifetime images of Early-, Mid- and Late S-phase DNA replication sites labeled with AlexaFluo 546. (b) Color-coded image of the lifetime distribution throughout cell nucleus. (c) Schematics of the FLIM approach to measure compaction of chromatin replicating at different S-phase windows. (d) Averaged fluorescence lifetime of AlexaFluo546 used to label DNA replication sites in Early-, Mid-, and Late S-phase. The error bars correspond to standard deviations. Data indicate the higher refractive index, and thus higher compaction density for gene-poor chromatin domains replicating in Late S-phase compared to gene-rich chromatin replicated in Early S-phase.
Credit
by Svitlana M. Levchenko, Artem Pliss, Xiao Peng, Paras N. Prasad and Junle Qu
Usage Restrictions
Credit must be given to the creator.
License
CC BY