Figure 2. (IMAGE)

Kanazawa University

Figure 2.

Caption

Internalization of aptamer into A549 cells is indispensable for CYP24 inhibition and enhancing 1, 25-D3 mediated cell-growth inhibition. (A) Internalization of Aptamer into A549 cells. A549 lung cancer cells were treated with 500 nM aptamer Apt-7 and had their membranes stained green (Wheat Germ Agglutinin (WGA) Conjugates). Confocal microscopy revealed the intracellular signal, derived from internalized aptamer (red fluorescence, Cy3). (B) Apt-7 inhibits the CYP24-mediated catabolism of 1,25-D3 in A549 cells. After 18 h of aptamer treatment, 1, 25-D3 metabolite conversion ratio and relative CYP24 activity was determined by HPLC. (C) Apt-7 enhances the anti-proliferative function of 1, 25-D3 in A549 cells.

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Kanazawa University

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