Genetic defense breakthrough: Plants repurpose stomatal genes to fend off herbivores

Ikoma, Japan—Throughout evolution, plants have continuously adapted to survive in changing environments. Apart from complex structural changes, plants have also developed various defense strategies against herbivores, including tougher protective layers, thorns, and chemical deterrents. Delving deeper into the evolution of defense mechanisms, a research team led by Assistant Professor Makoto Shirakawa from Nara Institute of Science and Technology (NAIST), identified a surprising genetic adaptation in the Brassicales plant order. In these cruciferous plants—including cabbage, mustard, and wasabi—genes originally used for gas exchange have been repurposed for defense.

The researchers uncovered the unique mechanism behind this evolutionary adaptation. Their findings were published online on February 24, 2025 and published in Volume 11 of the journal Nature Plants on March 01, 2025. The research team included Tomoki Oguro, Nobutoshi Yamaguchi, and Toshiro Ito from NAIST; Shigeo S. Sugano from National Institute of Advanced Industrial Science and Technology; Shohei Yamaoka and Takayuki Kohchi from Kyoto University; Yasunori Ichihashi from RIKEN Institute; Atsushi Takemiya from Yamaguchi University; and Takamasa Suzuki from Chubu University.

According to the study, FAMA, a protein primarily responsible for regulating gene expression for gas exchange, serves a dual role for the cruciferous plants. Beyond controlling stomatal (tiny pores for gas exchange) guard cells, FAMA also helps to produce myrosin cells—the specialized structures that store mustard oil compounds. So, when a plant is damaged, these compounds create a sharp, pungent taste that repels herbivores.

“We identified a specific gene called WASABI MAKER (WSB), which is directly activated by FAMA and is the key trigger for the development of myrosin cells,” shares Dr. Shirakawa. “When we studied the plants without WSB, we found that these defense cells failed to form, confirming its essential role in myrosin cell production.”

Additionally, the researchers identified another gene called STOMATAL CARPENTER 1 (SCAP1), which is also a target for FAMA. This gene collaborates with WSB in regulating guard cell development, but its role in myrosin cell formation appears to be secondary.

Evolutionary analysis suggests that these genetic pathways originally helped regulate stomatal development but were later repurposed for defense in Brassicales. “This discovery is particularly interesting because it highlights how gene repurposing allows plants to develop new survival strategies without evolving entirely new genes,” adds co-author Toshiro Ito.

This remarkable discovery offers promising avenues for improving crop yield. Modifying key genetic regulators like FAMA could help enhance the chemical defense in crops and vegetables, avoiding pest damage. Additionally, since FAMA also controls gas exchange, it can be optimized for efficient uptake of carbon dioxide in plants.

Moving forward, the researchers aim to uncover the mechanism of how plants have evolved to produce such a diverse range of specialized cells. Highlighting the significance of their research, Dr. Shirakawa concludes, “Beyond offering new insights for crop improvement strategies, we believe our future work will help answer one of biology’s most fundamental questions: How have plants achieved such remarkable diversity with a limited number of genes?”

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Resource

Title: Co-option and neofunctionalization of stomatal executors for defence against herbivores in Brassicales

Authors: Makoto Shirakawa, Tomoki Oguro, Shigeo S. Sugano, Shohei Yamaoka, Mayu Sagara, Mai Tanida, Kyoko Sunuma, Takuya Iwami, Tatsuyoshi Nakanishi, Keita Horiuchi, Kie Kumaishi, Soma Yoshida, Mutsumi Watanabe, Takayuki Tohge, Takamasa Suzuki, Yasunori Ichihashi, Atsushi Takemiya, Nobutoshi Yamaguchi, Takayuki Kohchi, and Toshiro Ito

Journal: Nature plants

DOI: 10.1038/s41477-025-01921-1

Information about the Plant Stem Cell Regulation and Floral Patterning Laboratory can be found at the following website: https://bsw3.naist.jp/ito/

About Professor Makoto Shirakawa from Nara Institute of Science and Technology, Japan

Dr. Makoto Shirakawa is an Assistant Professor at Nara Institute of Science and Technology (NAIST) in Ikoma, Japan. He earned his PhD from Kyoto University, Japan, and specializes in plant biology, molecular biology, cell biology, genome editing, and botany. His key research includes CRISPR/Cas9 applications in Marchantia polymorpha, studies on Arabidopsis thaliana, and plant development. For his remarkable contributions, he is recognized among Japan's top scientists in plant cell and developmental biology.

About Nara Institute of Science and Technology (NAIST)

Established in 1991, Nara Institute of Science and Technology (NAIST) is a national university located in Kansai Science City, Japan. In 2018, NAIST underwent an organizational transformation to promote and continue interdisciplinary research in the fields of biological sciences, materials science, and information science. Known as one of the most prestigious research institutions in Japan, NAIST lays a strong emphasis on integrated research and collaborative co-creation with diverse stakeholders. NAIST envisions conducting cutting-edge research in frontier areas and training students to become tomorrow's leaders in science and technology.

Website: https://www.naist.jp/en/