Naile Dame-Teixeira of the University of Brasilia in Brasilia, Brazil will present an Interactive Talk presentation, “Anacardic Acids are Potential Therapeutics for Root Caries”, on Wednesday, June 22nd, 2022 at 9:45 a.m. China Standard Time (UTC+08:00) during the “Cariology Research-Microbiological Studies/Biofilm IV” session.
The study aims to assess how Anacardic Acids (AAs) impact oral bacteria, from an antibacterial and anti-collagenolytic perspective, as well as their biocompatibility with dental pulp stem cells. Two hemi-synthetic saturated AA-cashew nutshell derivative compounds were selected (LDT11 and LDT409). Bacteriostatic activity was tested against Streptococcus mutans R9 and Veillonella parvula ATCC17745. Antimicrobial capacity against S. mutans biofilms was investigated using a collagen-coated Calgary Biofilm Device (CFU and Live/Dead Confocal). Collagenases from Clostridium histolyticum, Porphyromonas gingivalis and S. mutans were used to assess anti-collagenolytic activity. Biocompatibility of both compounds with Human dental pulp stromal Cells (HDPSCs) was investigated in 3 different donors (DREC ethical approval 251121/HA/336).
AA inhibited the growth of S. mutans and V. parvula, as well as partially inhibited bacterial collagenases (>5μg/mL). LDT11 (100μg/mL) inhibited 96% of collagenase activity. AA treatment was associated with odontoblast-like morphology, which was observed after 24h of treatment. LDT11 at 50 ug/mL concentration had bacteriostatic activity against S. mutans and V. parvula, antimicrobial activity against mature S. mutans biofilms as well as anti-collagenolytic activity against bacterial collagenases. It was biocompatible with HDPSCs, stimulating cells proliferation and differentiation.
The International Association for Dental Research (IADR) is a nonprofit organization with over 10,000 individual members worldwide, with a Mission to drive dental, oral and craniofacial research to advance health and well-being worldwide. To learn more, visit www.iadr.org.