VDR regulates autophagy via the MAPK/ERK and PI3K/AKT/MTOR pathways. (IMAGE)
Caption
Endometrial epithelial cells were transfected with either negative control siRNA or VDR siRNA, followed by RNA extraction after 48 h. Transcriptomic differential expression was detected using next-generation sequencing.
(A) Heatmap.
(B) KEGG analysis of differentially expressed mRNAs between the VDR silencing and negative control groups.
(C–E) Endometrial epithelial cells were transfected with negative control siRNA (NC), si-VDR-1, si-VDR-2, OE-VDR (VDR-overexpressing lentiviral vector), or NC (VDR-overexpressing control).
Proteins were extracted 48 h later. Western blot analysis was performed to detect the expression levels of p-ERK1/2, ERK1/2, p-AKT, AKT, p-mTOR, and mTOR.
(F, G) Western blot analysis was conducted to measure the levels of p-ERK1/2, ERK1/2, p-AKT, AKT, p-mTOR, and mTOR in endometrial tissues from patients with IUA and those with normal endometrial tissues.
The experiments were conducted in triplicate, and representative results are presented.
Error bars indicate the standard error. The symbols ∗ and ∗∗ denote p < 0.05 and p < 0.01, respectively.
Credit
Hongtao Zhu, Bo Yang, Hui Wang, Ping Nie, Xixi Wu, Ming Yong, Xingwei Jiang, Jianguo Hu
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