VDR uterine conditional knockout mice showed an endometrial fibrosis phenotype. (IMAGE)
Caption
(A) PCR was used to identify wild-type (WT) and VDR knockout homozygous mice.
(B) Immunohistochemistry was employed to assess VDR expression in both WT and VDR knockout (KO) homozygous mice.
(C) Western blot analysis was conducted to assess VDR expression in wild-type (WT) and VDR knockout (KO) homozygous mice. The experiment comprised three groups, namely, WT, VDR-KO, and VDR KO + rapamycin, with 5 mice per group. Rapamycin (1 mg/kg per mouse) was administered via intraperitoneal injection 3 times a week for 4 weeks.
(D, E) Masson staining was used to assess fibrosis levels across the three groups.
(F, G) Immunohistochemistry was employed to assess the expression of ATG7, LC3, P62, COL1, CDH1, and CDH2 in the endometrial tissues across the three groups of mice. The experiments were conducted in triplicate, and representative results are presented. Student’s t-test was used for continuous variables between two groups (n = 3).
Error bars indicate the standard error. The symbols ∗ and ∗∗ denote p < 0.05 and p < 0.01, respectively. Scale bar: 100 μm.
Credit
Hongtao Zhu, Bo Yang, Hui Wang, Ping Nie, Xixi Wu, Ming Yong, Xingwei Jiang, Jianguo Hu
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