GXYLT2 knockdown suppressed Wnt/β-catenin signaling pathway. (IMAGE)
Caption
(A) A TOP/FOPFlash luciferase reporter assay in GXYLT2-knockdown 293T cells. Firefly luciferase activity from TOPFlash or FOPFlash plasmid was normalized by Renilla luciferase activity from pRL-TK plasmid.
(B) Western blotting analyses for β-catenin phosphorylation and downstream genes of Wnt/β-catenin signaling pathway in HGC-27 (with serum starvation for 48 h), MKN1, and MKN45 cells with GXYLT2 knockdown.
(C) Immunofluorescence staining for β-catenin localization (green) in GXYLT2-knockdown HGC-27 cells with serum starvation for 48 h. DAPI (blue), nuclei staining. Scale bar, 10 μm.
(D) The β-catenin expression level in the nucleus and cytoplasm detected by western blotting analysis in HGC-27 (with serum starvation for 48 h).
(E) Immunohistochemistry staining for GXYLT2 and β-catenin proteins in GC specimens from our own cohort. Representative images of GC samples with intestinal or diffuse subtype were shown. Scale bar, 25 μm; scale bar in inset, 10 μm.
(F, G) Immunohistochemistry score correlations between GXYLT2 and nuclear localized β-catenin protein in the intestinal (F) and diffuse (G) subtypes from GC samples of our own cohort. Data were presented as mean ± standard deviation of three independent experiments. p-values were calculated with one-way ANOVA (A). ∗∗∗p < 0.001. GXYLT2, glucoside xylosyltransferase 2; GC, gastric cancer
Credit
Jiale Yang, Jiajun Wu, Ziqiang Chen, Xiangyun Hou, Xiaojing Li, Zhaorui Liu, Kai Yin, Tao Pang, Ruimin Huang, Jun Yan
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