Next-generation biliary niche-on-a-chip models can be fully characterised using an antibody stripping-based mIF protocol. (IMAGE)

First Hospital of Jilin University

Next-generation biliary niche-on-a-chip models can be fully characterised using an antibody stripping-based mIF protocol.

Caption

 (A) Whole-scan image of a biochip membrane prior to cell fixation, showing cellular nuclei stained with Hoechst 33342 and hepatocytes derived from a reporter transgenic (actin-CFP) mouse. (B) Single-channel representations from sequential mIF performed in the biliary niche-on-a-chip, showing multiple cell populations: desmin+ cells represent hepatic stellate cells, albumin+ cells represent hepatocytes, LYVE-1+ cells represent ECs, CD68+ cells represent liver macrophages and CK7+ cells represent liver intrahepatic cholangiocytes. (C) Merged images derived from different markers acquired during sequential mIF within the same biochip, either with all or a subset of markers displayed as merged images. The fluorophore conjugated to each secondary antibody used here is indicated in brackets on the single channel images. DAPI, 4′,6-diamidino-2-phenylindole; ECs, endothelial cells; mIF, multiplex immunofluorescence.

Credit

By Bianca Franco Leonardi, Guo Yin, Natalja Amiridze, et al.

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Credit must be given to the creator.

License

CC BY

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