Sequential multiplex immunofluorescence and CytoPrixm software overviews. (IMAGE)

First Hospital of Jilin University

Sequential multiplex immunofluorescence and CytoPrixm software overviews.

Caption

 (A) Generic workflow of multiplex immunostaining applied to the phenotypic characterisation of cells in formalin-fixed, paraffin-embedded tissues, illustrating the sequential steps from sample preparation to the acquisition of final analytical results. (B) CytoPrixm software component diagram. The pipeline is organised into three functional blocks: processing modules (bright blue), inputs (green) and outputs (yellow and purple). Solid arrows indicate data flow, while dashed arrows represent configuration or weight dependencies. Created with BioRender.com. Ab, antibody; DAPI, 4′,6-diamidino-2-phenylindole; NGS, normal goat serum; ON, overnight; PBS-T, phosphate-buffered saline containing 0.1% Tween 20; R-CNN, region-based convolutional neural network; RT, room temperature.

Credit

By Bianca Franco Leonardi, Guo Yin, Natalja Amiridze, et al.

Usage Restrictions

Credit must be given to the creator.

License

CC BY

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