Stat3 mediates the pro-fibrotic effects of Xbp1s in Ang II-infused mice (IMAGE)
Caption
(A) Cardiac fibroblasts (CFs) were isolated from Xbp1s Tgfl/fl mice and infected with an adenovirus carrying Cre to overexpress Xbp1s. Then, Stat3 expression was knocked down. Relative mRNA levels of α-SMA and Collagen III (n = 6). (B) These Xbp1s-overexpressed CFs were also subjected to a Stat3 inhibitor, and relative mRNA levels of α-SMA were detected (n = 5). (C) These Xbp1s-overexpressed CFs were also infected with an adenovirus carrying a mutant Stat3 promoter, and mRNA levels of α-SMA were detected (n = 5). (D) Western blotting analysis was performed to analyze the phosphorylation of Smad3 in CFs isolated from Xbp1s mfKO mice (n = 6). (E–G) Xbp1s mfTg were bred with Stat3 conditional knockout mice. Fibrotic area and cell area were detected in the indicated groups (n = 6). (H) Ejection fraction (EF) was detected in these groups (n = 8). Data are shown as mean ± standard error of the mean, and analyzed using one-way ANOVA followed by Tukey post hoc test. ∗P < 0.05 versus the matched control.
Credit
Yiming Dong, Hongyu Su, Peipei Zheng, Zekai Tao, Zhengyu Chen, Peiheng Han, Li Zhang, Yuan Liu
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