Molecular mechanisms of psiTPTE22-HERV-mediated tumor suppression (IMAGE)
Caption
(A) Heatmap of differentially expressed genes (DEGs; |fold change| ≥ 1.5 and FDR <0.05) identified by RNA sequencing, with functional annotations for top hits (|fold change| ≥ 2). (B) GO biological processes altered by psiTPTE22-HERV overexpression as identified by over-representation analysis. (C) KEGG pathways enriched by DEGs as identified by GSEA. (D) Western blotting analysis of PI3K/AKT/mTOR pathway components (PI3K, AKT, p-AKT, and mTOR), EIF4EBP1, p-EIF4EBP1(Thr70), and EIF4E. (E) Pearson's correlations of psiTPTE22-HERV expression with EIF4EBP1/EIF4E mRNA, EIF4EBP1/EIF4E protein, and p-EIF4EBP1(Thr70) protein levels in gastric tumors of the TCGA cohort. (F) Cytoplasmic-specific reduction of EIF4E by psiTPTE22-HERV, as evidenced by western blotting of cytoplasmic and nuclear fractions. (G) Immunofluorescence staining confirmation of EIF4E modulation by psiTPTE22-HERV.
Credit
Fei Xu, Mengwen Zhang, Suzhan Zhang, Yao Zeng, Shu Zheng, Jessie Qiaoyi Liang
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