Creating 3D-IHI nanoscaffold using biodegradable nanomaterials. (IMAGE)
Caption
a) A schematic diagram showing the 3D-IHI nanoscaffold could enhance chondrogenic differentiation of BMSC through a synergy between N-cadherin and FAK-mediated pathways. b) The strong interactions between MnO2 NTs and functional groups commonly existing in ECM proteins effectively supported cell attachment as demonstrated via SEM image. c) Bicinchoninic acid assay indicated the enhanced absorption toward gelatin from MnO2 nanotube compared to control groups. d) The MnO2 nanotube-templated assembly method significantly enhanced cell-matrix interaction as demonstrated through the up-regulated expression patterns of the FAK gene. e) Representative immunostaining images showing the improved chondrogenesis of BMSC in the BMSC-IHI nanoscaffold group compared to the control groups. Scale bar: 50 μm. f-h) The expression of chondrogenic genes, including SOX9 (f), Aggrecan (g), and Col-II (h) were characterized via qRT-PCR measurement.
Credit
©Science China Press
Usage Restrictions
Use with credit.
License
Original content