Three genes upregulated and Treg numbers decreased in canine massive hepatocellular carcinoma
image: Through RNA sequencing, abnormal genes associated with liver cancer in dogs were examined.
Credit: Osaka Metropolitan University
The mechanisms underlying the development and prognosis of hepatocellular carcinoma (HCC) differ between dogs and humans. Although genetic alterations can cause carcinogenesis, the genetic alterations in canine HCC may differ from those observed in human HCC. In humans, various signaling pathways, oncogenes, and tumor suppressor genes are associated with HCC oncogenesis. Immune cells have been reported to be associated with prognosis. In canine HCC, genes related to oncogenesis and prognosis remain unclear. Therefore, an Osaka Metropolitan University-led research team used RNA sequencing to comprehensively evaluate abnormally expressed genes and infer immune cell composition in canine HCC.
Fourteen dogs histopathologically diagnosed with HCC and four dogs with normal livers (NL) were included in this study. No HCC recurrence or metastasis was observed. A total of 260 differentially expressed genes were identified in dogs with HCC compared to those in NL dogs. Among them, 119 were downregulated and 141 were upregulated in HCC. Among the ontology and oncogenic signature gene sets, PRC1, CDCA3, and CDC20 were upregulated in the top 20 genes. Regulatory T cells (Treg) levels were decreased in the livers of dogs with HCC compared to those in normal livers.
PRC1, CDCA3, and CDC20 were upregulated and identified as candidate oncogenic genes in canine massive HCC. Downregulation of Tregs may be associated with prognosis in canine massive HCC.
###
About OMU
Established in Osaka as one of the largest public universities in Japan, Osaka Metropolitan University is committed to shaping the future of society through “Convergence of Knowledge” and the promotion of world-class research. For more research news, visit https://www.omu.ac.jp/en/ and follow us on social media: X, Facebook, Instagram, LinkedIn.