Differential expression analysis of single-cell RNA-Seq data in monocytes across R-ISS stages. (IMAGE)
Caption
A: The t-SNE plot shows eight different subclusters of monocytes (M1–M8). B: Split plot showing the single landscape of monocytes split by R-ISS stage. C: The bar plot displayed relative contributions of monocytes from M1 to M8 in every patient. D: Heat map showing the representative upregulated genes (top 10) in M1 to M8. The top 10 genes in M4 are marked. E: Heat map showing differentially expressed genes of monocytes involved in R-ISS stages. F: Violin plots representing heterogeneity in the expressions of CCL3, CCL3L1 and CXCL2 in plasma cells from normal and different R-ISS stages. G: Heat map presenting obviously activated regulons in each cluster. The four regulons (JUND, JUN, FOSB and ATF3) enriched in M4 are marked. H: Heat map showing that diverse regulons were notably expressed in each R-ISS stage. The four marked regulons originated mainly from M4. I: The network shows representative regulons in M4 containing binding motifs, transcription factors and associated target genes.
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