Agriculture

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16-Dec-2025

 

A ‘one-pot’ assay of or rapid portable identification of genotypes I and II African swine fever viruses

KeAi Communications Co., Ltd.
Peer-Reviewed Publication

Researchers in China developed a 'one-pot' Cas12i3/Cas13d-based assay, designated OBServe.v2, to discriminate between ASFV genotype I and II. This method demonstrates complete concordance with the PCR-sequencing method within 45 min.

Journal

Journal of Integrative Agriculture

Funder

National Natural Science Fund for Distinguished Young Scholars of China, Shandong Provincial Key R&D Program, China, Biological Breeding-Major Projects, Institute of Zoology, Chinese Academy of Sciences and Institute of Animal Science, Chinese Academy of Agricultural Sciences, Special Project of Longhu Laboratory, China, National Natural Science Foundation of China, National Key Research and Development Program of China