Gene delivery via nanotubes changes fish sex to boost aquaculture yield
Peer-Reviewed Publication
Updates every hour. Last Updated: 23-Dec-2025 16:11 ET (23-Dec-2025 21:11 GMT/UTC)
Functionalized single-walled carbon nanotubes (SWCNTs) effectively delivered the amh/amhy plasmid into all-female mandarin fish via immersion. At 40 mg/L, plasmid DNA and transcripts were detected in gonads within 7-14 days. By 60-120 days, some fish developed masculinized gonads with downregulated foxl2/cyp19a1a and upregulated amh/dmrt1. The study demonstrates SWCNTs as a viable gene delivery tool in fish and confirms the crucial role of amh/amhy in sex determination via the amhrII/smads pathway activating dmrt1.
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