Three-dimensional visualization of nanoplastic distribution in the neonatal mouse brain
National Institute for Environmental StudiesPeer-Reviewed Publication
A joint research team from the National Institute for Environmental Studies (NIES), The University of Osaka, and Waseda University has developed a method to visualize the three-dimensional distribution of nanoplastics in the neonatal mouse brain without tissue sectioning. By combining tissue clearing with light‑sheet fluorescence microscopy, the researchers observed more extensive accumulation of 50 nm polystyrene nanoplastics than larger 500 nm particles in multiple organs—including the brain, intestine, and kidneys—following neonatal oral administration. Whole‑brain imaging revealed the widespread distribution of 50 nm particles, with relatively higher signals in the thalamus and brainstem. This study establishes a foundational three‑dimensional imaging platform for investigating nanoplastic biodistribution during early development. Of note, environmental exposure levels and health risks are not directly assessed in the study, which focuses on the development of a key methodological tool for research in the field. This research article is scheduled for publication on June 29, 2026 in the Journal of Hazardous Materials Advances (Elsevier).
- Journal
- Journal of Hazardous Materials Advances
- Funder
- Japan Society for the Promotion of Science, Japan Society for the Promotion of Science, The Environment Research and Technology Development Fund, The Environment Research and Technology Development Fund