Harnessing non-matched illumination for dark-field-like contrast in live-cell imaging
Peer-Reviewed Publication
Updates every hour. Last Updated: 16-Jan-2026 04:11 ET (16-Jan-2026 09:11 GMT/UTC)
The geometry of standard multi-well cell culture plates restricts oblique illumination angles, preventing matched illumination condition required for accurate tomographic reconstruction. To overcome this limitation, researchers developed the DF-FPDT technique, which leverages non-matched illumination and harnesses it as an intrinsic mechanism for dark-field-like contrast enhancement. By selectively updating high-frequency components using Phase Transfer Function (PTF) filtering, DF-FPDT effectively addresses low-frequency loss, enabling high-resolution, high-contrast live-cell imaging and dynamic screening, making DF-FPDT a powerful tool for biomedical research under realistic laboratory conditions.
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